BMM202L

School of Engineering \ Biomedical Engineering

1

2

Compulsory

Turkish

BMM 102

Department of Biomedical Engineering Department

In this course, students are taught fundamental biology and molecular biology techniques, which are also practically applied. Topics covered include concentration calculations, microscope components and working principles, DNA isolation from human cells, determination of DNA concentration and quality using spectrophotometry, polymerase chain reaction (PCR), genotype determination using restriction endonuclease digestion and agarose gel electrophoresis, cell culture, enzymatic detachment and passaging of surface-dependent cells, cell counting using a hemocytometer, protein lysate extraction, protein separation using polyacrylamide gel electrophoresis (PAGE), and protein detection through Western blotting.

1. Altınışık, M., Çözeltiler ve konsantrasyon hesabı, ADÜTF Biyokimya AD, 2008.
2. Bonifacino JS,Dasso M, Harford JB, Lippincott-Schwartz J, Yamada KM, Current Protocols in Cell Biology, Wiley, New York,2000.
3. Davis JM. Basic Cell Culture, 2nd edition; IRL, Oxford, 2002.
4. Hücre Kültürü Kursu Notları, Süleyman Demirel Üniversitesi, Isparta, 2011.
5. MSUM Biochem & Biotech, Counting Cells with a Hemocytometer, 2006.
6. TUBA Bilimler Akademisi Ulusal Açık Ders Sistemi “Ökaryotik Hücre Kültürü”
7. Klug WS, Cummings MR, Spencer CA, Genetik (C. Öner, S. Sümer, R. Öner, A. Öğüş, L. Açık, Çev.), Sekizinci Baskı, Palme Yayıncılık, 2009.
8. Temizkan G, Yılmazer S, Öztürk M, Arı Ş, Ertan H, Sarıkaya AT, Arda A. Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitabevleri, 2008.

The objectives of the BMM202L course are to provide general information on the rules and safety measures to be followed in the cell and molecular biology laboratory, to teach the examination of cell structure through experimental studies, the use of light microscopy, general principles and essential considerations of cell culture, the evaluation of experimental results, the ability to present experimental results in written form, and to foster teamwork skills.

1. Learning the fundamental principles regarding rules and safety measures in the cell and molecular biology laboratory.
2. Gaining theoretical knowledge on molecular techniques such as the use of light microscopy, DNA isolation, PCR, gel electrophoresis, and cell culture.
3. Developing practical skills to perform these experiments and understanding how to apply this knowledge to analyze and solve current engineering problems.
4. Gaining experience in teamwork, conducting literature research, maintaining laboratory notebooks, and writing reports.

Week 1: Introduction to cell and molecular biology laboratory and concentration calculations
Week 2: Microscope components and working principles. Estimation of human hair thickness using a microscope, examination of leaf and epithelial cell morphology
Week 3: DNA isolation from human cells, determination of DNA concentration and quality using spectrophotometry
Week 4: Polymerase Chain Reaction (PCR)
Week 5: Genotype determination using restriction endonuclease digestion and agarose gel electrophoresis
Week 6: Introduction to cell culture. Enzymatic detachment and passaging of surface-dependent cells. Cell counting using a hemocytometer, protein lysate extraction
Week 7: Protein separation using polyacrylamide gel electrophoresis (PAGE) and protein detection through Western blotting
Week 8: -
Week 9: -
Week 10: -
Week 11: -
Week 12: -

• Final 30 %
• Quizzes 30 %
• Homeworks 10 %
• Lab Notebook 10 %
• Performance 20 %